Download MendeleyCRYSTAL STRUCTURE OF AS-ISOLATED F295LMUTANT OF THREE-DOMAIN HEME-CU NITRITE REDUCTASE FROM RALSTONIA PICKETTII
Petchyam, N., Antonyuk, S.V., Hasnain, S.S.To be published.
9FOM | pdb_00009fom
CRYSTAL STRUCTURE OF AS-ISOLATED F295L MUTANT OF THREE-DOMAIN HEME-CU NITRITE REDUCTASE FROM RALSTONIA PICKETTII
- PDB DOI: https://doi.org/10.2210/pdb9FOM/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Ralstonia pickettii
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2024-06-12 Released: 2025-06-25
- Funding Organization(s): UK Research and Innovation (UKRI)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.16 Å
- R-Value Free: 0.139 (Depositor), 0.139 (DCC)
- R-Value Work: 0.114 (Depositor), 0.114 (DCC)
Starting Model: experimental
View more details
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Copper-containing nitrite reductase | 468 | Ralstonia pickettii | Mutation(s): 1 Gene Names: nirK, D7S62_04310 EC: 1.7.2.1 |  | |
UniProt | |||||
Find proteins for I6NAW4 (Ralstonia pickettii) Explore I6NAW4 Go to UniProtKB: I6NAW4 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | I6NAW4 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| HEC (Subject of Investigation/LOI) Query on HEC | D [auth A] | HEME C C34 H34 Fe N4 O4 HXQIYSLZKNYNMH-LJNAALQVSA-N |  | ||
| CU (Subject of Investigation/LOI) Query on CU | B [auth A], C [auth A] | COPPER (II) ION Cu JPVYNHNXODAKFH-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.16 Å
- R-Value Free: 0.139 (Depositor), 0.139 (DCC)
- R-Value Work: 0.114 (Depositor), 0.114 (DCC)
Space Group: H 3
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 128.257 | α = 90 |
| b = 128.257 | β = 90 |
| c = 86.194 | γ = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| Aimless | data scaling |
| xia2 | data reduction |
| Coot | model building |
| REFMAC | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-06-25 Deposition Author(s): Petchyam, N., Antonyuk, S.V., Hasnain, S.S.
| Funding Organization | Location | Grant Number |
|---|---|---|
| UK Research and Innovation (UKRI) | United Kingdom | BB/N013972/1 |
| UK Research and Innovation (UKRI) | United Kingdom | BB/L006960/1 |
Revision History (Full details and data files)
- Version 1.0: 2025-06-25
Type: Initial release
