Download MendeleyFunctional Characterization and Structural Insights into SrUGT76G1-4 from Stevia Rebaudioside Reveal a Residue Critical for the Regioselectivity and efficient Rebaudioside M synthesis
Wang, Y., Li, T., Yin, H.To be published.
8ZI6 | pdb_00008zi6
Crystal structure of SrUGT76G4 in complex with UDP-glucose
- PDB DOI: https://doi.org/10.2210/pdb8ZI6/pdb
- Classification: TRANSFERASE
- Organism(s): Stevia rebaudiana
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2024-05-13 Released: 2025-05-21
- Funding Organization(s): Not funded
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.351 (Depositor), 0.350 (DCC)
- R-Value Work: 0.268 (Depositor), 0.270 (DCC)
- R-Value Observed: 0.272 (Depositor)
Starting Model: in silico
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This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| UGT-glycosyltransferase 76G4 | 458 | Stevia rebaudiana | Mutation(s): 0 EC: 2.4.1 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| UPG (Subject of Investigation/LOI) Query on UPG | D [auth A], F [auth B] | URIDINE-5'-DIPHOSPHATE-GLUCOSE C15 H24 N2 O17 P2 HSCJRCZFDFQWRP-JZMIEXBBSA-N |  | ||
| PO4 Query on PO4 | G [auth C] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| IPA Query on IPA | E [auth A] | ISOPROPYL ALCOHOL C3 H8 O KFZMGEQAYNKOFK-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.351 (Depositor), 0.350 (DCC)
- R-Value Work: 0.268 (Depositor), 0.270 (DCC)
- R-Value Observed: 0.272 (Depositor)
Space Group: P 31
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 111.78 | α = 90 |
| b = 111.78 | β = 90 |
| c = 91.77 | γ = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| SCALA | data scaling |
| MOSFLM | data reduction |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
| Funding Organization | Location | Grant Number |
|---|---|---|
| Not funded | -- |
Revision History (Full details and data files)
- Version 1.0: 2025-05-21
Type: Initial release
